The role of the Center for Research on Genomics and Global Health (CRGGH) laboratory is to manage the center's large biorepository and to design and perform all key "wet-lab" experiments related to CRGGH activities. Located on the fifth floor of Building 50, Room 5531 (separate from the CRGGH office suite also on the NIH main campus), the laboratory is managed and maintained by Ayo Doumatey, Ph.D. and staffed by a research associate (Lin Lei) with expertise in chemistry and molecular biology as well as genetic and genomic techniques. The laboratory is also home to many of CRGGH's trainees.
While space is dedicated for CRGGH wet-lab activities, research projects also make use of a number of shared resources offered by NHGRI, including the Division of Intramural Research core instruments and services. Below are brief descriptions of just a few of the many cellular and molecular techniques currently employed by the CRGGH lab:
- Bioassay/ Biomarker analysis
- The CRGGH laboratory uses a number of technologies to measure the levels of selected biomolecules in serum (e.g., adipokines) or other biological fluids. The major methods include commercially available ELISA kits and the Bio-Plex® Suspension Array System (Bio-RAD).
- Customized genotyping using mid-throughput platform, namely the Sequenom® technology, which allows multiplexing for up to 32 SNPs in a single reaction across thousands of individual samples.
- Drug metabolite array such as the Affymetrix DMET™ chip, which supports genotyping of about 1936 variations in 225 genes associated with the absorption, distribution, metabolism and excretion (ADME) of pharmaceutical drugs.
- Gene expression
- RT-PCR for genes of interest using Applied Biosystems chemistry on BIO-RAD instruments (MyiQ).
- Whole genome gene expression array, such as the GeneChip® Human genome U133 array from Affymetrix.
- DNA and RNA extraction from peripheral blood cells and multiple tissue sources
- Automated extraction (EZ1 instrument and consumables from QIAGEN)
- Manual extraction using commercially available kits.
- Whole genome amplification (WGA)
The CRGGH laboratory also does a large number of sample preparations for clinical assays and ongoing collaborative activities.
Last Updated: February 5, 2015